HESI Biology Practice Questions — Free Sampler (2026)

PreNursePrep has 155 Biology questions for the HESI A2, each written to the current blueprint and each with a rationale explaining why the right answer is right. The free sampler gives you 40 of them, timed and scored like the real exam, with no card and no sign-up.

  • 155 Biology questions in the bank
  • 30 on the real exam · 25 minutes
  • Rationale on every question

3 sample Biology questions

Drawn from the live HESI A2 bank. Answers and rationales open in the practice player.

  1. Question 1Cellular respiration and photosynthesis

    A cell is placed in an anaerobic (oxygen-free) environment. Which of the following correctly describes the sequence of events that allows the cell to continue producing ATP through fermentation after glycolysis?

    • ANADH donates its electrons to pyruvate, regenerating NAD+ so glycolysis can continue
    • BThe Krebs cycle runs in reverse to produce additional ATP without oxygen
    • CThe electron transport chain switches to carbon dioxide as the final electron acceptor
    • DPyruvate enters the mitochondrial matrix and is fully oxidized to generate ATP
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  2. Question 2DNA/RNA, protein synthesis, heredity (Punnett squares)

    A DNA template strand has the sequence 3'-TACGGATCC-5'. What is the corresponding mRNA sequence produced during transcription?

    • A5'-AUGCCUAGG-3'
    • B3'-AUGCCUAGG-5'
    • C5'-TACGGATCC-3'
    • D5'-ATGCCTAGG-3'
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  3. Question 3Cell structure and organelles

    A biology student examines a cell under a microscope and observes a large central vacuole, a cell wall, and chloroplasts. Which type of cell is the student most likely viewing?

    • AAnimal cell
    • BBacterial cell
    • CPlant cell
    • DFungal cell
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